Proteasome



Proteasome (PRTS) are large protein complexes which degrade unneeded proteins into small polypeptides. The 26S PRTS is composed of a central 20S core particle which contains 4 stacked rings each with several members and two 19S caps. The core particle two outer rings contain 7 alpha subunits which form the PRTS gate. The two inner rings contain 6 beta subunits with protease activity. The 19S cap is composed of a base with 10 proteins six of which are ATPases and a lid which contains 9 proteins which binds polyubiquitin. The images at the left and at the right correspond to one representative Proteasome, i.e. the crystal structure of yeast Proteasome (3gpt).

3D Structures of Proteasome
Update June 2011

20S particle
3mfe, 3mi0, 2fhg – Mt20S core – Mycobacterium tuberculosis

3gpt, 3gpw, 1g0u – y20S core - yeast

1ryp - y20S core (mutant)

2zcy – y20S+syringolin

3mka, 3hfa – Mt20S core (mutant)

3c91, 3c92, 1pma – Ta20S core - Themoplasma acidophilum

3h4p – 20S core – Methanocaldococcus janaschii

2h6j, 1q5r – Re20S core (mutant) – Rhodococcus erythropolis

1q5q - Re20S core

1j2q – Af20S core - Archaeoglobus fulgidus

1iru – 20S core - bovine

20S+activator protein
1vsy, 1l5q – y20S core+ Blm10

1z7q, 1fnt – y20S core+PA26

3ipm, 3jrm, 3jse, 3jtl, 1ya7, 1yar, 1yau - Taa-PRTS chains A-G+ Tab-PRTS chains H-N+PA26 chains O-U

20S+inhibitor
3hye, 3gpj, 3dy3, 3dy4, 3e47, 3d29, 3bdm, 2zcy, 2gpl, 2fak, 2f16, 1jd2, 1g65, 3mg0, 3mg4, 3mg6, 3mg7, 3mg8 – y20S core+inhibitor

3h6f, 3h6i, 3hf9, 2fhh, 3krd – Mt20S core+inhibitor

3nzj, 3nzw, 3nzx - y20S + TMC-95A mimic ligand

Partial 20S
2ku1, 2ku2 – Taa-PRTS chains A-G

3fp9 – MtATPase chains A-L

1j2p – Af α subunit

2jay – Mt β subunit

2kqz, 2kr0 – hPRTS ubiquitin receptor domain

Partial 26S
1p9c – hPRTS subunit 4 C-terminal – human

1p9d - hPRTS subunit 4 C-terminal + HHR23A ubiquitin-like domain

1uel - hPRTS subunit 4 C-terminal + HHR23A ubiquitin-like domain - NMR

1yx4 - hPRTS subunit 4 – NMR

1yx5, 1yx6, 2kde, 2kdf - hPRTS subunit 4+ubiquitin – NMR

2o95, 2o96 - hPRTS subunit 7

3kw6 - hPRTS subunit 8

2krk - hPRTS subunit 8 – NMR

1qym, 1uoh - hPRTS subunit 10

1tr4 - hPRTS subunit 10 – NMR

2dvw, 2dwz - mPRTS subunit 10 + hPRTS subunit 6B – mouse

3aji - mPRTS subunit 10 + subunit 4

2x5n - PRTS subunit RPN10 VWA domain – fission yeast

2z4d - yPRTS subunit RPN13

2z59 - mPRTS subunit RPN13+ubiquitin